Effects of total iridoid glycosides of Picrorhiza scrophulariiflora against non-alcoholic steatohepatitis rats induced by high-fat and high-sugar diet through regulation of lipid metabolism

更新时间:2023-05-28

【摘要】Objective: To investigate the therapeutic effect of total iridoid glycosides of Picrorhiza scrophulariiflora(TIGP) on non-alcoholic steatohepatitis(NASH).Methods: SD rats were fed with high-fat and high-sugar diet for 8 weeks to establish NASH. TIGP were given orally at doses of 20, 40 and 80 mg/kg/d for 4 weeks. Triglycerides assay(TG), total cholesterol(TC), low density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), aspartate aminotransferase(AST), alanine aminotransferase(ALT), fasting plasma glucose(FPG), fasting insulin(FINS), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), chemokine-1(MCP-1), leptin(LEP) in serum were tested. TG, TC, superoxide dismutase(SOD), malondialdehyde(MDA), and free fatty acid(FFA) in liver tissue were determined by colorimetric methods. Steatosis of hepatocytes and inflammation was performed by pathological examination.Results: The results showed that TIGP significantly decreased TC, TG and FFA in liver tissue, increased SOD activity, decreased MDA content, decreased serum levels of TG, TC, HDL-C/LDL-C, ALT, AST, GLU,HOMA-IR, TNF-α and LEP, and in addition, improved steatosis of liver cells compared to NASH.Conclusion: TIGP had anti-fatty liver effect against NASH rats induced by high-fat and high-sugar diet. Its mechanism was related to the regulation of lipid metabolism and reduction of insulin resistance, through inhibition of oxidative stress and inflammation.

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